Dr Ibidapo Williams

• Qualifications:PhD
• Position:Senior Research Fellow
• Department:Faculty of Environment and Technology (FET) HAS - Applied Sciences
• Telephone:+4411732 86186
• Email:Ibidapo.Williams@uwe.ac.uk

About me

I have a doctorate degree from De Montfort University where I studied Molecular Biology and Toxicology. During my time at De Montfort University, I created several expression plasmids (CYPS for drug metabolism, 1A2 3A4, 2C9 2C19 2D6 and other variants), these plasmids were used to established expression systems in mammalian, insect and yeast cells. These expression systems were taken up for commercial research by a company, CYP Design LTD. Also during this time, I developed a crude technique for using whole cells in the biotransformation of natural compounds and other potential therapeutic target drugs. E.g Chrysin to Baiceilin (https://doi.org/10.1021/acs.jafc.7b02690) etc. Led the project of establishment of the point of care or home use bio-sensor for vitamin D using the whole cell's system. Since joining  UWE, Bristol in 2018, I developed new assay models that have been deployed in numerous research areas and projects:  the recent work was the measurement  CASPACES- biomarkers involve in cell death- Apoptos (https://doi.org/10.2139/ssrn.4070649) as  part of supervisory support role for post graduate researcher. My current projects are : Biomarkers in COPD (co-investigatorinnovate UK), (primary investigator-antibiotic sensor monitoring development (VC-ECR Award), Propofol biosensor devolopment for  continuous monitoring (co-investigator-Innovate UK) etc.

Area of expertise

• Excellent background in molecular cloning techniques (DNA amplification and cloning, vector design, Ligation of insert + Vector, DNA sequence analysis, mutagenesis, gel purification, Electrophoresis, archiving of samples, sample preparation for transfections into a mammalian cell, and purity check). I have clone more than 40 plasmids that are have been expressed in mammalian and yeast cells.
• Development of Expression system used in Bio-sensor for home or point of care for patient use.
• Vast experience in primer design, vector modification, promoter modification, tandem for expression up-regulation, and PCR.
• The design of plasmid Maps constructs for protein expression.
• Expert in writing, criticizing both academic and industrial journals
• Actively collaboration with big pharma for drug discovery potential (Presently working on the finding of metabolites products from AZD2014 (AstraZeneca compound drug), also I have generated plasmids that are presently available for use (depends on interest).
• I have an excellent Team spirit and Train team members when needed

• Design of Experiment (DOE)
• Process Modelling. Monitoring & Statistical Data Analysis Using SAS-JMP 7.0, Molecular Devices
• Process optimization, scale-up, and validation Studies for Bio-manufacturing of phase I-II products, including Cell Therapeutics and Proteins and enzymes(Yeast-Microsomes)
• Freeze drying (Lyophilisation of enzymes) protein products, dissolution,  
• Mammalian Cell Culture, Cell Transfection, and Microbial Fermentation
• Batch and Fed-Batch Stirred Tank Bioreactor Operation and Troubleshooting
• Membrane Filtration, PCR.
• Metabolites and Protein Analysis and Characterization using LC-MS-Mass Spec, HPLC-Agilent, SDS-PAGE, Coomassie and Silver Staining, Western Blot, ELISA. UV-Spec, TLC, Densitometry Analyses, etc.
• IP and commercialization 
  

Publications

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